Molecular And Phenotypic Analysis Of Pseudomonas Aeruginosa Strains Isolated From Chronic Wound Infection

Abstract

After Escherichia coli and Staphylococcus aureus, P. aeruginosa is regarded as the third nosocomial pathogen. Additionally, it is the leading cause of hospital infections, particularly in patients who have burns, immunosuppression, or intubation due to cystic fibrosis (CF). One of the most dangerous and resistant to drugs is Pseudomonas aeruginosa. In many parts of the world, P. aeruginosa resistance to antipseudomonal medications has been steadily rising over time. The aim of the current study is to isolate P. aureginosa stains from chronic wound infections and analyze them molecularly and phenotypically. In the study total 100 samples were collected from burn unit patients with mild to severe burn wounds exhibiting burn infection symptoms from district Abbottabad. The isolated bacteria were then characterized on the basis of their morphology and microscopic analysis. All strains are classified and characterized as P. aureginosa by pale colonies on MacConkey media. Biochemical characterization was conducted to characterize isolates of pathogens. The biochemical test results show that isolates show negative results towards methyl red, and positive results towards catalase and oxidase. Resistance profiles were evaluated through the use of antibiotic susceptibility testing. DNA extraction was conducted using ladder of 1500 bp the results show P. aureginosa band size is of 700bp. The phylogenetic analysis results confirm the isolated bacterial pathogen as P. aureginosa strains.

Keywords: P. Aureginosa, Chronic Wound, Antimicrobial Susceptibility