Detection of Ganeo Species (Trematoda) of the Viscera of Hoplobatrachus Tigerinus in District Swabi, Pakistan

Abstract

The purpose of this study is to identify the frog intestinal trematodes that were collected in District Swabi, Khyber Pakhtunkhwa, Pakistan, using their morphological traits. The study has screened 30 Anurans (order) frogs in this study. Two genus Ganeo species of parasite were retrieved out of these frogs. To have permanent slides, specimens were immobilized in F.A.A. (a solution of formalin, acetic acid and 50% alcohol in proportion of 6, 2 and 92). The slides were then dyed with acetocarmine and preserved with Canada balsam. Next, for the purpose of morphological identification Photomicrographs and line drawings were employed. The measurements were recorded in millimeters. Key features of Ganeo sp. Its main characteristics are described as follows: the body is oval in shape, the oral sucker is large in size to which the ventral sucker is small and the latter overlaps with lower testis. The testis has an almost oval form, whereas the ovary is rounded and longer than it is wide. The uterine coils make contact with the ovary. The Ganeo species has been extensively documented in frog hosts, particularly in Hoplobatrachus tigerinus. Both of the organisms that were recovered are intestinal trematodes. This is the initial detection of Ganeo sp. in the Swabi area. The discovery made in this study will assist researchers in identifying trematodes in frogs. The categorization of helminths in amphibians, and frogs in particular, requires further molecular research.